Antibody response of beef calves to experimental monovalent and multivalent inactivated bovine viral diarrhoea virus vaccines as measured by indirect ELISA method
2011
Kurćubić, V., Faculty of Agronomy, Čačak (Serbia) | Petrović, T., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Đoković, R., Faculty of Agronomy, Čačak (Serbia) | Ilić, Z., Faculty of Agriculture, Zubin Potok (Serbia) | Petrović, M.D., Faculty of Agronomy, Čačak (Serbia)
The objective of this study was to determine the immunogenic properties of two experimental inactivated (mono - and multivalent) vaccines containing BVDV type 1 reference strains (NADL, W1. - 162903, W2. - 172984, W3. - 173481, W4. - 179725) and one local field isolate derived from a calf suffering from mucosal disease (MD). Normal healthy beef calves (Simmental race) of mixed sex, 6 to 7 months of age, were divided into three experimental groups: ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated multivalent vaccine per animal (Group 1); ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated monovalent vaccine per animal (Group 2) and 9 unvaccinated calves (Control group C). Blood sera were obtained from immunized animals (standard procedure: on days 0, 14, 28, 42 and 56 post-immunization). The immune response to BVDV vaccine strains was assessed by the indirect ELISA method (Bovine Viral Diarrhoea Virus Antibody Test Kit BVDV HerdChek*BVDV Ab, Idexx Scandinavia AB), according to the producer's manual. BVD virus specific antibodies were first detected 42 days after primary immunization and 14 days after secondary immunization in both experimental groups of beef cattle and persisted until day 56 of the experiment. The statistically highly significant differences (of 99%) between Group 1 and Group 2 animals on days 42 and 56 of the experiment suggest considerably higher immunogenicity of the monovalent vaccine used to immunize Group 2 cattle.
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