Marker-assisted selection for waxy and opaque-2 genes in waxy corn breeding
2010
Angkana Poniyom(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) E-mail:[email protected] | Choosak Jompuk(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Witith Chai-Arree(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Bunyarit Sinkangam(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Wassamon Mongkol(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy)
This study was to transfer the opaque-2 gene from the field corn QPM line to waxy inbred lines by backcross breeding program. The F1-hybrid was made between waxy and QPM lines. The waxy lines, Kwi1 and Kwi9 were used as a female parent and Q53 (QPM line) was used as male parent. The BC1F1 in each cross was obtained by backcross method. In the BC1F1 plants, an SSR marker (phi057) Fergason was applied to detect the heterozygous plants (O2o2). Twenty-two plants of the Kwi1xQ53 and thirty-one plants of the Kwi9xQ53 were selected of opaque-2. Then, these plants were self-pollinated to make the BC1S1. By the markers of phi057 and phi022 (waxy), the homozygous recessive plants (wxwxo2o2) were obtained and were self-pollinated to make BC1S2. Finally, two lines of Kwi1xQ53 and three lines of Kwi9xQ53 were selected. These lines gave more tryptophan content in endosperm than the waxy parent, but they still gave high amylopectin as the waxy parent.
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