Increasing of tryptophan in waxy corn by Opaque-2 gene and marker-assisted selection
2010
Wassamon Mongkol(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) E-mail:[email protected] | Choosak Jompuk(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Rungsarid Kaveeta(Kasetsart University, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy) | Bunyarit Sinkangam(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Angkana Poniyom(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy)
Objective of this study was to increase of the tryptophan content in an endosperm of waxy corn by the opaque-2 gene from the quality protein maize (QPM) and using the phi057 and phi022 marker-assisted selected in the S1 progenies until S4 progenies. The waxy corn inbred lines, Kwi1 and Kwi9 were directly crossed with PopII no. 53 (QPM). The results showed that S1 progenies of Kwi1 x PopII no.53 and Kwi9 x PopII no. 53 were detected for O2O2:O2o2:o2o2 of 17:49:8 and 28:71:2 by the phi057, respectively. Then, the o2o2 plants were selected of both crosses. Furthermore, the marker phi022 was applied to detect the waxy gene. Eight selected lines for waxy-opaque genes were obtained and then self-pollinated to advance the generation three times. Ten S4 progenies containing with o2o2wxwx (double homozygous recessive) were selected. Furthermore, the amylopectin content in endosperm of selected lines was analyzed and ranged 97.72-98.18 percent. Moreover, the tryptophan in endosperm content was about 0.063-0.145 percent.
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