In vitro screening of various Jatropha curcas seeds for high protein and low toxic curcin
2010
Sansaneeyaporn Kittikajhon(Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) | Sittiruk Roytrakul(National Science and Technology Development Agency, Bangkok (Thailand). National Center for Genetic Engineering and Biotechnology) | Nuanchawee Wetprasit(Ramkhamhaeng University, Bangkok (Thailand). Faculty of Science. Department of Biotechnology) | Sunanta Ratanapo(Kasetsart University, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) E-mail:[email protected]
A toxin, curcin was simple purified from seed kernel of Jatropha curcas L. (KUBP 33) by precipitating out of the other proteins with acidic pH. The soluble purified curcin showed relative molecular weight of 29 kDa by SDS-PAGE and N-glycosidase activity towards 28s rRNA from rabbit reticulocyte lysate. The amount of curcin in seeds of the total 153 accessions of J. curcas collected, from various regions (38 provinces) of Thailand were detected and quantitated by Western blotting probed with antibody to curcin. One of the three accessions which did not have the toxic curcin, KUBP19 (from Roi-Et, Amphoe Nong-Phok) showed the highest protein level (16.07 percent of the dry weight). The J. curcas accessions from various regions were classified into four groups based on the percentage of curcin content with dry weight of the seed kernel.
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