Određivanje sadržaja mikotoksina zearalenona u dječijoj hrani na bazi mlijeka i žita primjenom ELISA metode
2010
Jasmina Tahmaz | Ahmed Smajić | Mersiha Alkić | Nermina Đulančić
The main objective of this paper was to examine the possibility of zearelenone contamination of powdered milk based baby food samples. Fusarium moulds (generally F graminearum, F. culmorum, F. crookwellense) produce mycotoxin zearalenone by their own metabolic activity. It is proved on experimental animals that zearalenone is toxic for reproductive system and because of that zearalenone is typical estrogenic mycotoxin. Correlation between zearalenone presence in food and human disease is not quite confirmed. However, it is believed that zearalenone caused early puberty in children in Porto Rico between 1978. and 1981. Children with noticed preterm sexual characteristics had a high level of zearalenone in blood. Most of the baby food products are cereal based. They can contain maize, wheat, oat or barley kernel, flour, grits, or flakes and because of that there is a possibility that cereal based baby food may be contaminated with micotoxins like zearalenone. ELISA method was used for quantitative determination of zearalenone content in 24 samples of milk based baby food. Analyses were carried out in the Institute for Dairy Science of Justus Liebig University in Giessen, Germany. Detection limit was 4 ng/g, and quantitative determination was performed by spectrophotometer with wave length 450 nm. Four samples were positive on zearalenone, yet none of them contained zearalenone concentration higher than permitted limits. The highest level of 11,23 ng/g was found in one sample of baby food containing dried milk and whole wheat kernel.
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