Direct detection of Ehrlichia canis by PCR in the conjunctiva of a dog with bilateral anterior uveitis
2012
Walser-Reinhardt, L. | Matheis, F. | Spiess, B., Universitaet Zuerich, Zuerich (Switzerland). Departement fuer Pferde, Abteilung Ophthalmologie | Schaarschmidt-Kiener, D. | Forster, J.-L.
The following report describes the direct detection of Ehrlichia canis by real-time PCR in the conjunctiva of a 1-year-old female Maltese dog. After being imported from Brazil, the dog was presented because of anorexia, dehydration, fever and palpable mandibular lymph nodes. A few days later, the dog developed bilateral blepharospasm, photophobia and anterior uveitis. Monocytic ehrlichiosis was diagnosed by a positive PCR result and the detection of IgM and IgG antibodies. Because of the massive uveitis a conjunctival sample was taken with a cytobrush, which also tested positive for Ehrlichia canis DNA by real-time PCR. Only one week after starting treatment with systemic doxycycline and local anti-inflammatory and cycloplegic therapy the dog recovered from systemic and eye diseases. After therapy the follow-up examination revealed a full remission of clinical and hematological parameters and a negative PCR result.
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