Transcriptional profiles of inflammatory related cytokines in PK-15 cell cultures following infection with Porcine circovirus type 2 | PCV-2感染对PK-15细胞抗病毒相关因子转录时相的影响

Chinese. 了解猪圆环病毒2型（PCV-2）感染对PK-15细胞抗病毒相关因子转录时相的影响，探讨宿主-病毒之间的作用关系。运用荧光定量PCR技术，测定和分析PCV-2感染PK-15细胞后0(未接种病毒)，1，6，12，24，48，72 h时病毒DNA含量及细胞因子IFN-β、IFN-γ、IFNAR-1、IFNAR-2、MHC-Ⅰ、MHC-Ⅱ、MX1、NOS、RNaseL和IRF-3 mRNA转录水平的变化。PCV-2感染PK-15后1 h就可以检测到病毒DNA，在感染后24 h病毒开始大量迅速增殖，于感染72 h时达到最高峰。与0 h相比， PCV-2感染后IFN-β、IRF-3的表达量在感染12 h时显著增加，其他时间表达量下降；IFN-γ、MHC-Ⅱ的表达量在感染12 h时增加不明显，其他时间表达量下降；感染PCV-2后IFNAR-2、MHC-Ⅰ、MX1的表达量下降；RNaseL的表达量在感染12 h时显著减少，其他时间表达量增加，48 h时表达量增加显著；IFNAR-1的表达量在感染72 h时显著增加，其他时间表达量下降；未检测出NOS的表达。随着PCV-2病毒含量的增加，以上几种主要抗病毒因子的表达量不明显或有所下降，表明PCV-2感染PK-15细胞后可逃避机体的抗病毒作用机制。

English. The study was to investigate the host inflammatory responses and the host-PCV-2 interaction in particular antiviral cytokines responses when the Porcine Kidney-15 cells (PK-15) were infected with Porcine Parvovirus (PCV-2). After the Porcine Kidney-15 cells (PK-15) were infected with PCV-2, the viral DNA was measured and analyzed by real-time PCR, so were the transcript level of cytokines (IFN-β, IFN-γ, IFNAR-1, IFNAR-2, MHC-Ⅰ, MHC-Ⅱ, MX1, NOS, RNaseL and IRF-3). We found that the transcript levels of IFN-β,IRF-3 were significantly increased at 12 h postinoculation (p. i.), but decreased at other time. The transcript levels of IFN-γ, MHC-Ⅱ were slightly increased at 12 h p. i. and decreased at other time. The transcript levels of IFNAR-2, MHC-Ⅰ, MX1 were decreased. The transcript levels of RNaseL were significantly decreased at 12 h p. i. but increased at other time. The transcript levels of IFNAR-1 were significantly increased at 72 h p. i. but decreased at other time. With the increase of the content of the PCV-2, the expression quantities of the above several main antiviral factors were not obvious or declined which showed PCV-2 infection can evade the antiviral mechanism of PK-15 cells.