The effect of new chemical hybridizing agent SX-1 on Brassica napus L. in two ecological regions | 化学杂交剂SX-1对不同生态区甘蓝型油菜的杀雄效果

Chinese. 研究在不同生态区化学杂交剂SX-1对甘蓝型油菜的杀雄效果。在冬油菜区和春油菜区分别采用4个不同质量浓度的化学杂交剂SX-1,对YD66A和Cn10 2个甘蓝型油菜品种(系)进行2次喷施杀雄处理，第1次喷施在油菜最大花蕾的花粉母细胞进入单核期时进行，第2次喷施在第1次喷施后10～15 d内进行，以喷施等量清水为对照，研究在不同生态区喷施SX-1的杀雄效果，并探讨其对油菜生长发育、农艺性状和产量的影响。冬油菜区A2（6.0 mg/L+7.0 mg/L）和春油菜区B2（6.5 mg/L+7.0 mg/L）处理的YD66A和Cn10的全不育株率分别为：98.11%，100 %和100%，100%，且无药害反应，植株叶色变浅，茎秆微紫，生长轻微受到抑制，株高和主花序长度有所降低，单株角果数和单株产量略有增加。SX-1在冬油菜区以质量浓度6.0 mg/L+7.0 mg/L，在春油菜区以6.5 mg/L+7.0 mg/L，分别在油菜最大花蕾的花粉母细胞进入单核期时进行首次喷施，首次喷施后10～15 d进行第2次喷施，均可获得较好的杀雄效果。

English. The effect of new chemical hybridizing agent SX-1 on Brassica napus L. was studied in two different ecological regions. In order to study the influence of SX-1 on the rapeseed growth, inducing male sterility, agronomic traits and yield in the different ecological regions and the different mass concentrations, pure water selected was as control, chemical hybridizing agent SX-1 with four different mass concentrations were sprayed two times on rapeseed lines YD66A and Cn10 in winter rapeseed region and spring rapeseed region. The first spraying occurred when pollen mother cell of maximum bud blossom entered monocyte stage, and the second spraying was done 10-15 days later. Treated with A2 (6.0 mg/L+7.0 mg/L) and B2 (6.5 mg/L+7.0 mg/L) of SX-1 in two ecological regions, YD66A and Cn10 have the sterile rates of 98.11%, 100%, 100% and 100%, respectively. Moreover, the sterile plants have some good characteristics, such as no negative reaction to SX-1, leaf color becomes weaker, stem color is light purple, little inhibition for plant growth, plant height and the length of main inflorescence are reduced, the number of silique and yield per plant increase a little, and so on. With the concentration of (6.0 mg/L+7.0 mg/L) in rapeseed spring region and the concentration of (6.5 mg/L+7.0 mg/L) in rapeseed winter region, both sprayings of SX-1 have good effect on inducing male sterility.