Screening of Ferulic acid degrading actinomycetes and their degradation and inhibiting effect | 阿魏酸降解放线菌的筛选及其降解与拮抗效果研究

Chinese. 筛选用于降解西瓜根泌自毒物质阿魏酸的放线菌，研究其降解与拮抗效果。以127株放线菌为供筛菌株，采用液体发酵培养法筛选阿魏酸降解放线菌，并研究放线菌对阿魏酸的降解率及对西甜瓜枯萎病菌等病原菌的拮抗性。①从127株供试放线菌中筛选出了编号为25，28及Z30的3株放线菌，在纯培养条件下，以阿魏酸为惟一碳源时，25、28、Z30 3株放线菌对阿魏酸均有较好的降解效果，降解率分别为94.3%，92.6%和90.1%。②在以阿魏酸、淀粉为混合碳源时，3株放线菌对阿魏酸均有较强的降解作用，降解率最高达100%。③纯细胞培养时，3株放线菌对阿魏酸仅有微弱的降解效果。④3株放线菌对西甜瓜枯萎病菌有较强的拮抗作用。筛选的3株放线菌具有降解西瓜自毒物质阿魏酸及拮抗西甜瓜枯萎病菌的双重功能。

English. The experiment was to screen degrading actinomycetes of ferulic acid and to study the degradation and inhibitory effect of the actinomycetes. Liquid fermentation experiment was carried out to screen degradating actinomycetes of ferulic acid from 127 actinomyces strains and to study the degradation rate of ferulic acid by the actinomyces and their inhibiting effect on melon and cucumber fusarium wilt pathogen. ① The actinomycetes 25, 28 and Z30 were screened from 127 tested actinomycetes strains. Under pure culture conditions, the ferulic acid was the sole carbon source. The degradation rates of ferulic acid completed by the actinomycetes 25, 28 and Z30 were 94.3%, 92.6% and 90.1%, respectively. ② In a mixture of ferulic acid and starch as carbon sources, the degradation rate of ferulic acid completed by the three actinomycetes was 100%. ③ The degradation rate of ferulic acid by three actinomycetes as pure cell was low. ④ The three strains of actinomycets have good inhibiting effect on the melon and cucumber fusarium wilt. The degradation effect of ferulic acid by three actinomycetes and their inhibiting effect on the melon and cucumber fusarium wilt are good.