Effects of Pb 2+/Cd2+ on the number of microorganisms in the rhizosphere of winter wheat seedlings | Pb2+/Cd2+澶瀵瑰害骞艰归寰╃缇ゆ伴褰卞

Chinese. ㄧ借楠硅插害骞艰锛寰骞艰3 ㄣ7 ㄥ12 ㄦ讹堕圭郴锛杩琛归澹ょ剧嚎浜纭烘爱伴娴瀹缁琛ㄦ害骞艰3 ㄦ讹Pb2+/Cd2+瀵规归缁烘爱浜纭琛ㄧ涓烘捐(p0.05) 朵骞冲剁负20.41%~49.79%26.62%~76.99%87.15%~95.88%锛剧嚎伴富瑕琛ㄧ涓哄锛骞冲澧负0.31%~38.88%19.58%~113.64%锛浣寰╂绘板锛澶锋ф板锛/缁姣煎楂锛骞艰7 ㄦ讹剧嚎烘爱浜纭伴富瑕琛ㄧ涓哄璞★骞冲澧负18.16%~19.72%13.71%~24.59%2.09%~ 519.29%锛伴浣骞冲浣涓9.59%~39.46%锛缁伴ㄤ搴跺锛楂娴搴跺锛寰╂绘板锛澶锋ф颁富瑕琛ㄧ涓洪浣/缁姣奸浣骞艰12 ㄦ讹Pb2+/Cd2+澶涓缁伴捐(p0.01)澧锛剧嚎烘爱浜纭缁伴浣骞冲浣负12.05%~44.66%1.94%~15.51%12.44%~55.72%97.88%~99.00%锛寰╂绘颁富瑕琛ㄧ涓哄锛澶锋ф伴浣/缁姣兼捐浣(p0.01)Pb2+/Cd2+澶瀵瑰害骞艰归绫诲井╂伴寰╂绘板澶锋ф板/缁姣煎奖捐(p0.05)锛涓寰╁锋ф扮涓昏充剧嚎伴锛Pb2+/Cd2+瀵瑰害归烘爱浜纭伴褰卞瑙寰涓绋冲锛棰绀虹Pb2+/Cd2+姹℃灏褰卞舵归澹N 哄杞藉锛涓N 婧╃ㄧ涓绋冲

English. Pot experiments were conducted to culture winter wheat seedlings. The rhizosphere soil was collected to determine the number of bacteria, fungi, actinoplanes, nitrogen-fixing bacterial, and nitrosobacterial when seedlings had grown for three weeks,seven weeks, and twelve weeks, respectively. The number of bacteria, nitrogen-fixing bacteria, and nitrosobacteria in Pb2+ /Cd2+significantly(p0.05) decreased by 20.41%-49.79%, 26.62%-76.99%, and 87.15%-95.88%, respectively, and the number of fungi and actinoplanes increased by 0.31%-38.88% and 19.58%-113.64%, respectively at the third week. Moreover, the total number of microorganisms decreased and the diversity and the ratio of fungi / bacteria increased in Pb 2+/Cd2+ at the third week. When winter wheat seedlings grew for seven weeks, the number of actinoplanes, nitrogen-fixing bacteria, and nitrosobacterial mainly increased by 18.16%-19.72%, 13.71%-24.59%, and 2.09%-519.29%, respectively and that of fungi decreased by 9.59%~39.46%.Moreover, the number bacterial increased in low doses of Pb 2+/Cd 2+and decreased in high doses of Pb 2+/Cd 2+. At the same time,the total number of microorganisms increased and the diversity and the ratio of fungi/bacteria decreased in the stress of Pb2+ /Cd 2+.When winter wheat seedlings grew for twelve weeks in Pb2+ /Cd2+, the number of bacteria significantly(p0.01) increased, and the number of fungi, actinoplanes, nitrosobacterial, and nitrogen-fixing bacterial decreased by 12.05%-44.66%, 1.94%-15.51%,12.44%-55.72%, and 97.88%-99.00%, respectively. At the same time, the total of microorganism mainly increased, the diversity reduced, and the ratio of fungi/ bacterial significantly(p0.01) decreased. The effect of Pb2+ /Cd2+ on the number of rhizosphere microorganisms, diversities, and the ratio of fungi/bacteria was significant (p0.05) and the shift of the diversity was mainly due to the change of the number of actinoplanes. The irregular change of nitrosobacterial and nitrogen-fixing bacteria indicated that the nitrogen fixed capacity of soil in the rhizosphere of winter wheat seedlings was affected by Pb2+ /Cd 2+in the future. Moreover,the utilization of nitrogen resource was also affected.