Sequence difference of mitochondrial DNA control region and genetic differentiation of Cervinae in China | 涓介箍浜ㄧmtDNA跺哄宸堕浼
2011
Tu Jianfeng, Chinese Academy of Agricultural Science, Jilin(China), Institute of Special Economic Animal and Plant of Science | Xing Xiumei, Chinese Academy of Agricultural Science, Jilin(China), Institute of Special Economic Animal and Plant of Science | Xu Jiaping, Chinese Academy of Agricultural Science, Jilin(China), Institute of Special Economic Animal and Plant of Science
[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing, Combined with homologous sequences of others Cervinae downloaded from GenBank,then analyzed through biology software. [Conclusion] The lengths of their control region were 921~1072 bp, the nucleotide content of T, A, C, and G was 32.1%30.2%22.7% and 15.0% separately, genetic distance of each species ranged from 0.062~0.106, they belonged to level of inter-genus. the molecular phylogenetic tree indicated that red deer, skia deer and White-lipped Deer had close relationship, they constituted one branch with sambar deer, Eld's deer and elk deer constituted the second branch, and hog deer constituted the third branch alone. [Result] Hog deer and fallow deer should be incorporated in Cervus,divergence time were about 1.55~2.60 million years among Cervinae in China.
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