Effect of copper and zinc on accumulation of vitamin E in wheat embryo callus | 瀵瑰害芥浼ょ缁缁寸绱E绉疮褰卞
2011
Zhang Heng, Huaiyin Institute of Technology, Huaian, Jiangsu (China) | Xu Zhaotang, Huaiyin Institute of Technology, Huaian, Jiangsu (China) | Li Wenqian, Huaiyin Institute of Technology, Huaian, Jiangsu (China)
[Objective] The aim was to study the effect of the content of Copper and Zinc on the vitamin E accumulation of wheat embryo callus in the culture. [Method] The mathematical models were established to describe the growth kinetics and the vitamin E accumulation in wheat embryo callus cells. With the aim of getting the highest accumulation of secondary metabolites Vitamin E, the optimal combination of Copper and Zinc in medium was confirmed by testing. [Result] The results showed that the production of vitamin E in B5 medium reached the highest value with 2.0 mg/ml ZnSO4_H2O and 0.1mg/mL CuSO4_H2O. The fitting degrees of kinetic models of Vitamin E accumulation and cell growth were 97.53% and 95.60%, respectively, which indicated good nonlinear relationships. [Conclusion] Both Copper and Zinc could affect the accumulation of Vitamin E in wheat germ callus, and Copper showed more prominent effect than Zn. Synergism existed in low copper and zinc concentration, and the inhibitive effect enhanced with the increase of the concentrations.
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