Integratting the low temperature stress transcription factor bcice1 gene into rice improves cold tolerance | 转低温胁迫转录因子BcICE1基因水稻提高抗寒性

Chinese. 摘　　要：低温冷害是制约黑龙江省水稻安全生产最主要的气象灾害之一,严重影响其产量和品质。为提高寒地水稻抗低温冷害能力,本研究将从大白菜分离的低温胁迫转录因子BcICE1基因（GenBank登录号为HQ902162）构建到植物表达载体pCAMBIA1301上,利用农杆菌介导法导入粳稻品种龙粳24中。PCR、Southern杂交和RT-PCR检测结果表明,BcICE1基因已经成功整合到水稻基因组中,并正常表达。与对照相比,低温处理后超表达BcICE1基因的转基因株系存活率和脯氨酸含量明显增加,相对电导率和丙二醛含量积累速率明显下降,提高了抗低温胁迫能力。

English. Chilling damages severely affecting rice yield and quality, is one of the most important meteorological disasters restricting rice safety in production of Heilongjiang province. To improve tolerant capacities of rice in cold regions to low temperature stress, in this study, cold stress transcription factor BcICE1 gene （GenBank Accession No.： HQ902162） from Brassica pekinensis was constructed on plant expressing vector pCAMBIA1301 and transformed into Japonica rice Longjing24 by Agrobacterium-mediated transgenic technique. PCR, Southern blot and RT-PCR analysis indicated that BcICE1 gene had been successfully integrated into the genome of transgenic rice lines and was expressed normally. After low temperature stress treatment, compared to control, the transgenic lines with high expression levels of the BcICE1 gene had obvious higher survival rates and proline content. In the meantime, the accumulation rate of relative conductivity and malondialdehyde （MDA） content were much lower. Overexpression of BcICE1 gene improved low temperature stress tolerant capacities in transgenic rice lines.