Study on the coexistence of gnrh, ot, syn and s-100 protein in the same luteal cell of the mid phases of oestrous cycle in karakul sheep | GnRH、OT、SYN及S-100蛋白在卡拉库尔羊周期黄体细胞中的共存

Chinese. 目的研究卡拉库尔羊周期黄体中OT、GnRH两种生殖激素以及突触素（synaptophysin，SYN）、S-100蛋白两种DNES（dispersed or diffuse neuroendocrine system，DNES）细胞标志物在同一细胞中的共存。方法采用免疫荧光双标记法分别用OT、GnRH、SYN和S-100蛋白的单克隆抗体两两组合对黄体组织进行染色观察与分析。结果OT与GnRH、OT与S-100、OT与SYN、GnRH与S-100及GnRH与SYN 5种组合的免疫荧光染色双阳性细胞在黄体组织中广泛存在；在5种双阳性细胞两两之间的10种组合中，有9种组合的双阳性细胞间表现为高相关性（相关系数0.7以上）。结论发情周期卡拉库尔羊黄体组织中，广泛存在DNES细胞；OT和GnRH广泛存在于黄体的DNES细胞中，DNES细胞可能是OT和GnRH的分泌细胞，黄体细胞具有生殖内分泌细胞和DNES细胞的双重属性。

English. ObjectiveThe experiment was conducted to study coexistence of gonadotropin-releasing hormone (GnRH), oxytocin (OT), synaptophysin (SYN) and S-100 protein of dispersed or diffuse neuroendocrine system (dispersed or diffuse neuroendocrine system，DNES) in the same luteal cell. Method The corpus luteum was stained using pairwise combinations of OT, GnRH, SYN, and S-100 monoclonal antibody through double labeling immunofluorescence staining, respectively, at the mid phases of oestrous cycle of the Karakul sheep. Result Five kinds of immunofluorescence staining double positive cells combinations (OT and GnRH, OT and S-100, OT and SYN, GnRH and S-100, and GnRH and SYN) were respectively presented in luteal cell. In addition, the correlation analysis on 10 combinations of 5 kinds of double positive cells showed that there were 9 highly correlated combinations (r＞0.7). Conclusion This result reveals that there are DNES cells widespread in the same luteal cells，and there are OT and GnRH widespread in DNES cells. So, DNES cells may be the secretion cells of OT, and GnRH, and there are dual properties of both DNES cells and endocrine cells in luteal cells.