Effects of diets supplemented with nano-cuo and high level of zinc sulfate on copper and zinc contents of visceral tissues of chickens | 日粮添加高水平硫酸锌和纳米氧化铜对鸡脏器组织锌铜沉积的影响

Chinese. 目的研究高锌（硫酸锌）常铜（纳米氧化铜）日粮对鸡脏器组织铜锌含量的影响，探讨Zn2+与纳米氧化铜在消化道吸收时的互作关系。方法选取1日龄SPF白莱航蛋用公雏120只，随机分为4组，每组5个重复。1组为对照组，饲喂基础日粮（以纳米氧化铜的形式添加铜8 mg#8226;kg-1）；2―4组为高锌组，以ZnSO4#8226;7H2O的形式分别在基础日粮中添加锌250、500和1 000 mg#8226;kg-1。结果日粮添加锌250―1 000 mg#8226;kg-1，受试鸡肝、腺胃、十二指肠和空肠中锌和金属硫蛋白含量显著高于对照组（P＜0.01或P＜0.05），鸡十二指肠铜含量极显著高于对照组（P＜0.01）。日粮添加锌1 000 mg#8226;kg-1时，空肠铜含量极显著高于对照组和日粮锌添加组（锌添加量分别为：250和500 mg#8226;kg-1，P＜0.01）。试验至42 d，日粮添加锌500和1 000 mg#8226;kg-1时，鸡肝脏铜含量极显著高于对照组和日粮锌添加组（锌添加量为：250 mg#8226;kg-1，P＜0.01）。日粮锌水平与鸡肝脏、十二指肠、空肠和腺胃锌含量呈显著正相关（P＜0.05），与十二指肠、空肠铜含量呈显著正相关（P＜0.05），与肝脏铜含量呈正相关（P＞0.05），与十二指肠、腺胃和空肠金属硫蛋白含量呈正相关（P＞0.05）。肝脏金属硫蛋白含量和铜含量呈极显著正相关（P＜0.01）；十二指肠、空肠金属硫蛋白含量和铜含量呈正相关（P＞0.05）。结论日粮添加高水平硫酸锌没有拮抗鸡对纳米氧化铜的消化吸收，纳米氧化铜在消化道可能是以纳米颗粒的形式吸收。

English. Objective In order to discover the interaction between Zn2+ and nano-CuO in the digestive tract of chickens, an experiment was conducted to determine the effect of high zinc (ZnSO4#8226;7H2O) and normal copper (nano-CuO) diets on copper and zinc contents of visceral tissues in chickens.MethodOne hundred and twenty one-day-old White Leghorn SPF chickens were divided into 4 groups randomly, and five replicates for each group. The basal diet was supplemented with nano-CuO to supply the copper level ats 8 mg#8226;kg-1. The chickens of control group were fed with basal diet. The chickens of trial groups were fed with diets supplemented with ZnSO4#8226;7H2O to supply the zinc level at 250 mg#8226;kg-1, 500 mg#8226;kg-1 and 1 000 mg#8226;kg-1, respectively.ResultThe results showed that when the chickens were fed with diets containing zinc at 250 mg#8226;kg-1, 500 mg#8226;kg-1 and 1 000 mg#8226;kg-1, the zinc and metallotionein contents of liver, proventriculus, duodenum and jejunum were significantly higher than the control group (P＜0.01 or P＜0.05), and the copper contents of duodenum were significantly higher than the control group (P＜0.01). When the diets containing zinc at 1 000 mg#8226;kg-1 were fed to chickens, the copper contents of jejunum were significantly higher than the control group and when the diets containing zinc at 250 mg#8226;mg-1 and 500 mg#8226;mg-1 were fed to chickens (P＜0.01). On 42nd day, when the chickens were fed with diets containing zinc at 500 mg#8226;kg-1 and 1 000 mg#8226;kg-1, the copper contents of liver were significantly higher than the control group and when the chickens were fed with diets containing zinc at 250 mg#8226;mg-1 (P＜0.01). The zinc levels of the diets had a remarkable positive correlation with zinc contents of liver, proventriculus, duodenum and jejunum (P＜0.05), had a remarkable positive correlation with copper contents of duodenum and jejunum (P＜0.05), and had a positive correlation with copper contents of liver (P＞0.05). There was a positive correlation between zinc levels of the diets and the metallothionein contents of duodenum, jejunum and proventriculus (P＞0.05), a significant positive correlation between metallothionein and copper contents of the liver (P＜0.01), and a positive correlation between metallothionein and copper content of duodenum and jejunum (P＞0.05).ConclusionIt is concluded that the absorption of nano-CuO is not antagonized by high level of zinc of diets supplemented with ZnSO4#8226;7H2O in the intestines of chickens, the nano-Cuo can be absorbed in the form of nanoparticle.