Effects of Turnip mosaic virus（TuMV）on endogenous hormones and transcriptional level of related genes in infected non-heading Chinese cabbage | 芜菁花叶病毒对不结球白菜内源激素含量及代谢相关基因转录水平的影响

Chinese. 摘　　要：以不结球白菜感病品种‘矮脚黄’为材料,采用ELISA和Real-time PCR技术研究了芜菁花叶病毒（TuMV）侵染后不结球白菜内源生长素（IAA）、赤霉素（GA3）、脱落酸（ABA）和茉莉酸（JA）的动态变化以及内源激素相关基因的表达差异。结果表明：TuMV侵染后,植株发病症状的严重程度与IAA含量呈正相关;光敏色素相关蛋白基因PAP1对IAA的合成起负调控作用。处理植株体内GA3含量及其代谢相关基因,即原表皮因子基因PDF1表达量均明显低于对照植株,而ABA含量则随着接种时间的延长不断积累,始终高于对照植株。且发病症状越严重的植株中,GA3含量越低,ABA含量越高。处理植株JA含量和JA诱导基因,即营养贮藏蛋白基因Vsp1表达量在接种后均出现2次上升,且植株病症越严重,JA含量越低。TuMV侵染后,植株中IAA/ABA和GA3/ABA的值则明显低于对照植株,表明TuMV的侵染破坏了内源激素的平衡,干扰了植物的正常生长发育。

English. ELISA and Real-time PCR experiments were conducted for investigating the dynamics of indoleacetic acid（IAA）,gibberellic（GA3）,abscisic acid（ABA）and jasmonate acid（JA）,and the expression changes of endogenous hormone related genes in response to Turnip mosaic virus（TuMV）infection on susceptible variety of non-heading Chinese cabbage＇Aijiaohuang＇.The results showed that there was a positive correlation between the level of plant disease degree and the content of IAA in TuMV infected plants.Gene PAP 1 encoding phytochrome-associated protein 1 played a negative regulation role on IAA synthesis.The content of GA3 and related protodermal factor gene PDF1 expression in TuMV infected plants were considerably lower than that in control.While the content of ABA in infected plants accumulated continuously with the increase of inoculation time,which was always higher than that in control.The severer the disease,the lower the GA3 content was,and the higher the ABA content was.The content of JA and JA-inducible vegetative storage protein gene Vsp1 expression had two rises after inoculation,and the more serious symptoms the plants had,the lower the content of JA was.The ratios of IAA/ABA and GA3/ABA in infected plants were both significantly lower than those in control in response to TuMV infection,which indicated that TuMV infection disrupted the balance of endogenous hormones,and thus disturbed the normal growth and development of plants.