Genetic diversity analysis of wild auricularia auricula strains in daxing’anling area using srap markers | 大兴安岭地区野生黑木耳菌株SRAP的遗传多样性分析
2011
Liu Huajing, Northeast Agricultural University, Harbin(China) | Xu Xiuhong, Northeast Agricultural University, Harbin(China) | Jiang Tingbo, Northeast Agricultural University, Harbin(China)
Chinese. 【目的】对大兴安岭地区野生黑木耳菌株进行遗传多样性分析。【方法】利用PCR-SRAP体系，选出9对SRAP引物对18个野生菌株和6个栽培菌株DNA进行扩增，通过NTSYSpc软件对遗传多样性进行分析。【结果】通过PCR扩增，9对引物共扩增到90条条带，其中78条多态性条带，多态性比率为87.0%。平均每条引物扩增的条带数和多态性条带数分别为10.00条和8.67条。多态性信息含量(PIC)变化在0.051―0.918，平均为0.683，所揭示的基因型数平均为15.78个。聚类分析结果表明，遗传相似系数在0.63水平上，可分为5个类群。【结论】SRAP标记技术在栽培与野生菌株间都表现出明显的遗传差异性，此技术可用于遗传多样性的分析研究。
Show more [+] Less [-]English. 【Objective】Genetic diversity of wild Auricularia auricula strains in Daxing’anling area was analyzed. 【Method】Using PCR-SRAP system, 9 pairs of primers on 18 wild strains and 6 cultivated ones were selected in expansion, and through clustering method its genetic diversity was analyzed.【Result】By PCR amplification, 90 fragments were amplified. Polymorphic bands were 78 which accounted for 87.0% in the total amplified fragments. The number of amplified and polymorphic fragments was 10.00 and 8.67 per primer pair, respectively. The PIC (polymorphism information content) value of these markers varied from 0.051 to 0.918, averaging 0.683. The genotypes revealed by each marker were 15.78 in average. According to the analysis results, the similarity coefficient was at the level of 0.63. They were divided into 5 groups.【Conclusion】There is an obvious genetic diversity in wild Auricularia auricula strains and cultivated ones, this technique can be used to analyze the genetic diversity in the future study.
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