Screening of proteins Interacting with porcine calsarcin-2 by yeast two-hybrid techniques | 应用酵母双杂交技术筛选猪Calsarcin-2蛋白结合蛋白
2011
Xin Leilei, Chinese Academy of Agricultural Sciences, Beijing(China), Institute of Animal Science | Zhu Wenjuan, Chinese Academy of Agricultural Sciences, Beijing(China), Institute of Animal Science | Yang E, Chinese Academy of Agricultural Sciences, Beijing(China), Institute of Animal Science
Chinese. Calsarcins是钙调磷酸酶肌小节特异性结合蛋白家族，本实验旨在研究Calsarcin-2(CS2)在猪骨骼肌纤维形成和信号通路调控中的功能。应用酵母双杂交方法筛选猪骨骼肌组织中CS2蛋白结合蛋白，从猪骨骼肌文库中筛选出180个阳性克隆，经分析得到12个相互作用的蛋白，研究发现骨骼肌α-肌动蛋白1(ACTA1)，肌联蛋白(TTN)及钙调素1(CALM1)为CS2互作蛋白。ACTA1参与肌肉收缩过程，TTN调节肌小节动力传导及Z线装配，CALM1参与钙离子信号传导。结果表明，CS2在维持Z线结构稳定和参与钙离子信号传导方面起重要作用。
Show more [+] Less [-]English. To elucidate the function of calsarcin-2(CS2) in the formation of skeletal muscle fiber and signaling pathway regulation, we used yeast two-hybrid approach to look for CS2 interacting proteins. The porcine skeletal library screen gave 180 positive interactions, in which 12 interacting proteins were obtained. Bioinformation analysis found that actin alpha 1(ACTA1), titin(TTN) and calmodulin 1 (CALM 1) were CS2 interaction partners. ACTA1 involved in the process of muscle contraction, TTN regulated the dynamics conduction of sarcomere and Z disc assembly, CALM 1 paticipated in calcium signal transduction. These results indicate that CS2 is implicated in the transduction of calcium signals and maintaining the structure of Z disc.
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