Optimization of extraction process of flavonoids from Ginkgo biloba L. leaf_ | 银杏叶黄酮提取工艺的优化____
2011
Xue Zhibin, Liaoning School of Medicine, Jinzhou (China) | Cheng Wei, Liaoning School of Medicine, Jinzhou (China)
Chinese. ［目的］优化银杏叶总黄酮的提取工艺，为银杏黄酮的精制提供前提条件。［方法］采用紫外分光光度法测定银杏叶中总黄酮的含量；用单因素试验考察乙醇浓度、回流温度、提取时间对银杏黄酮收率的影响，优化提取条件。［结果］银杏叶中总黄酮含量为1.502 6 mg/ml；最佳提取条件：70%乙醇为提取剂，回流温度为80 ℃， 提取时间为3.0 h。［结论］该研究得到银杏黄酮最佳的提取工艺，为银杏叶黄酮的精制提供了前提条件。_
Show more [+] Less [-]English. ［Objective］ To optimize the extraction process of flavonoids from Ginkgo biloba L. leaf, so as to provide basis for the refining of Ginkgo biloba L. leaf flavonoids. ［Method］ Content of flavonoids was determined by UV spetrophotometry. By employing single factor test, effect of ethanol concentration, reflux temperature and extraction duration on yield of flavonoids from Ginkgo biloba L. leaf, optimizing the extraction conditions. ［Result］ The optimal extraction conditions were determined to be: extracting solvent of 70% ethanol, extraction temperature of 80 ℃, extraction duration of 3.0 h. Under the optimum extraction process, content of flavonoids in Ginkgo biloba L. leaf reached 1.520 6 mg/ml. ［Conclusion］ The optimal extraction conditions were obtained in this study, which provided basis for refining of Ginkgo biloba L. leaf flavonoids._
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