Determination on Ca2+ concentration in the waste liquid of L-Ornithine extraction with HPLC-ELSD_ | HPLC-ELSD法检测鸟氨酸提取废液中的Ca2+浓度_

Chinese. ［目的］为鸟氨酸发酵废液中Ca2+含量的测定提供参考。［方法］以七氟丁酸为柱中衍生试剂，采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法定量测定L-鸟氨酸发酵提取废液中Ca2+的含量。［结果］在反相C18色谱柱上，以浓度0.14%三氟乙酸（含0.08%七氟丁酸）水溶液-乙腈溶液（84∶16，V∶V）作为流动相，能有效地检测Ca2+含量，检出限为Ca2+浓度0.000 2~0.001 6 mol/L。其相应的浓度（Y）与峰面积（X）呈线性关系，线性方程为Y=7×10-10X-3×10-8，相关系数为1.000 0。Ca2+的平均回收率为99.83%~100.3%，相对标准偏差为0.61%~1.01%。［结论］该方法精密度高，准确度好，稳定性高，能简便、快速、准确地测定鸟氨酸发酵液中Ca2+的含量。

English. ［Objective］The purpose of the research was to provide reference for determining Ca2+ content in the waste liquid of L-ornithine fermentation.［Method］ With heptafluorobutyric acid as derivation reagent in column，the Ca2+ content in the waste liquid of L-ornithine fermentation and extraction was determined quantitatively by high performance liquid chromatogram-evaporative light scattering detector.［Result］ With 0.14% trifluoroacetic acid (0.08% heptafluorobutyric acid) aqueous solution-acetonitrile solution (84∶16，V∶V) as mobile phase，the Ca2+ content could be determined effectively using reverse phase C18 chromatographic column and its detection limit was 0.000 2~0.001 6 mol/L. The corresponding concn. (Y) and peak areas (X) showed linear relationship，their linear equation was Y=7×10-10X-3×10-8 and its correlation coefficient was 1.000 0.The average recovery of Ca2+ was 99.83%-100.3% and its relative standard deviation was 0.61%~1.01%.［Conclusion］ This method had high precision，accuracy and stability and the Ca2+ content in the waste liquid of L-ornithine fermentation could be determined simply，rapidly and accurately by this method._