Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping
2011
Patzak, J., Chmelarsky Inst., Zatec (Czech Republic) | Matousek, J., Akademie Ved, Ceske Budejovice (Czech Republic). Ustav Molekularni Biologie Rostlin
The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect a better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in Humulus lupulus. They represent 25 gene loci with 1,268 EST sequences. They were used to characterize 11 hop samples and to cross-amplify in Humulus japonicus. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.
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