Bean polygalacturonase-inhibiting protein expressed in transgenic Brassica napus inhibits polygalacturonase from its fungal pathogen Rhizoctonia solani
2012
Akhgari, A.B., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) | Motallebi, M., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic) | Zamani, M.R., National Inst. of Genetic Engineering and Biotechnology,Tehran (Iran Islamic Republic)
Polygalacturonase-inhibiting proteins (PGIPs) selectively inhibit polygalacturonases (PGs) secreted by invading plant pathogenic fungi. The objective of the present research was to clone and introduce the pgip2 gene from Phaseolus vulgaris cv. Goli, with antifungal potential, into Brassica napus (R line Hyola 308) via Agrobacterium tumefaciens mediated transformation. Here we used a transgenic overexpression approach in order to investigate the inhibitory activity of the PGIP on the PG from Rhizoctonia solani. PGIP expression was determined in the functional inhibition assays against fungal PGs. Crude protein extracts prepared from transgenic canola leaves were found to inhibit the R. solani PG from 29% to 37% as compared to untransformed plants. The putative transgenic canola lines harbouring the pgip2 gene encoding polygalacturonase-inhibiting proteins were identified by polymerase chain reaction and Southern blot analysis.
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