Molecular fingerprinting of sorghum (Sorghum bicolor L. Moench) germplasm using simple sequence repeat markers
2010
Reddy, S. V. R. | Khanna, V. K.
SSR markers were used to study genetic diversity in sorghum. Fourteen diverse sorghum accessions were used for the present study. A set of 6 SSR specific primer pairs were used for PCR amplification. A total of 34 bands were amplified all of which were polymorphic and there were 3 unique bands. Accessions GFS-4 and UPFS-38 were very similar with a value of 0.728 whereas GFS-5 and FSH 92079 showed the lowest similarity value of 0.384. Accessions from IARI and NRCS were related to each other with a similarity value of 0.515 on an average. Accessions from Pantnagar and HAU were distantly related whereas accessions from GAU and ProAgro were similar to each other. The information content of the SSR data was high. The data from just one SSR locus allows the accessions to be uniquely identified. These SSRs can provide data that are useful to support genetic conservation.
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