Assessment of genetic relation of Indian isolates of four predatory fungi through RAPD markers
2010
De, S. | Sanyal, P.K. | Pan, D. | Bera, A.K. | Bandyopadhyay, S. | Pal, S. | Mandal, S C | Sarkar, A.K. | Patel, N.K. | Bhattacharya, D | Das, S.K.
The polymerase chain reaction (PCR) method was used to differentiate 4 commonly occurring deuteromycetes (fungi imperfecti), viz. Paecilomyces lilacinus, Verticillium chlamydosporium, Duddingtonia flagrans and Arthrobotrys oligospora using short sequence oligonucleotide primers. A total of 146 amplicons were identified in the genomic DNA of 4 fungi. Maximum genetic similarity was found in between Paecilomyces lilacinus and Verticilium chlamydosporium (39.96%) while Duddingtonia flagrans and Verticilium chlamydosporium showed the least (22.5%). Though the fungi are placed in the same group (fungi imperfecti) the results indicate the status of their distinct genotype at molecular level.
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