Development of rapd/scar marker for yellow mosaic disease resistance in blackgram
2011
Prasanthi, L. | Reddy, B.V.Bhaskara | Rani, K.Rekha | Devi, R.Mehala | Geetha, B. | Prasad, Y.Siva | Reddy, K.Raja
A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.
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