Identification of different forms of bacterial citrus canker agent and its Distribution and Host range in Iran
2009
Alizadeh Aliabadi, Ali, Iranian Research Institute of Plant Protection
Citrus bacterial canker (CBC) is one of the most important disease of citrus which caused by Xanthomonas citri subsp. citri (Xcc). To investigate the variability of Xcc, a collection of twenty five strains were isolated from Fars, Hormozgan, Kerman and Sistan-va-Baluchestan provinces of Iran and assessed phenotypically and genetically. These strains were similar in biochemical properties. Based on host range determination strains were divided into two groups; the first group was pathogenic on Mexican lime (Citrus aurantifolia), citrumelo (Poncirus trifoliata × C. paradisi), citrange (C. sinensis × P. trifoliata) and sour orange (C. aurantium) varieties whereas the second group was pathogenic on Mexican lime merely. Profile of cellular soluble proteins analyzed by sodium dodecyl sulphate-polyacryamid gel electrophoresis (SDSPAGE) and did not reveal any considerable differences among strains. Genetic diversity analyses were performed using two marker systems; repetitive polymerase chain reaction (rep-PCR) and random amplified polymorphic DNA (RAPD). The results of this research showed that two primers, ERIC 1R and 232, with the highest marker index, resulted in the most genetic variability among strains. The cluster analysis by the band patterns of the most primers allowed the discrimination of strains from Sistan-va-Baluchestan province as different group so it was concluded that geographical origin of these strains is differed from strains isolated from other provinces. Key words: RAPD, rep-PCR, citrus bacteria canker
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