Screening of sugar beet pollinator genotypes resistant to rhizomania using molecular marker
2011
Norouzi, P. | Mahmoudi, S.B. | Yaghoubi, B. | Orazizadeh, M.R. | Koohestani, M.S.
Rhizomania disease is the most important disease of sugar beet in Iran and some other parts of the world, and plays a significant role in decreasing sugar yield. The best approach to combat with this disease is the use of resistant varieties. In order to use these genes in breeding programs, tagging these genes with molecular markers is necessary. In order to identify the dominant homozygous plants resistant to Rhizomania within 67 sugar beet genotypes including S1, S2 and F2 populations, amounting to 1530 field or greennhouse grown single plants, a repulsion-phase RAPD marker, named PN3 linked to Rhizomania resistance was used. Genomic DNA was extracted from the leaf samples taken from the single plants. In the next step, primers related to PN3 marker were tested on single plants DNAs by RAPD-PCR method. Amplified products were separated by gel electrophoresis, stained with ethidium bromide, observed using gel documentation device and scored according to the presence and absence of marker band. According to the marker data, percentage dominant homozygous plants were found to be between 0 to 92%. Therefore, it seems PN3 marker after converting to SCAR specific marker can be utilized to screen rhizomania resistant pollinator breeding lines and populations originated from Rz1 source.
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