morphological and physiological studying
2006
Zarghami, Reza | Iran nejad, A | Dabir ashrafi, O
Cryopreservation technique is of a great importance in long-term preservation of plant germplasms. This techique is useful for plant preservation provided not inducing morphological and physiological changes in plants. Morphological and physiological changes in plantlets of two potato varieties, Agrya and Marfona, preserved in freezing and normal conditions were studied in this research. Twenty-three morphological characteristics were assessed using a descriptor with a scale of 0 to 9 for each trait. Free proline, soluble carbohydrates, soluble proteins and a and b chlorophyll content were measured for physiological studies. The results indicated a general homology for morphological traits of Agrya and Marfona plantlets preserved in freezing condition compared to non-freezing condition. The plants studied under various preservastion conditions revealed no significant difference for assessed physiological characteristics excepting free proline. Content of free proline in shoots prerserved in freezing condition increased significantly incomparison with non-freezing condition which this increment may be due to cold and oxidative stresses induced in plants.
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