Coenzyme Q∧10 Production by Sphingomonas sp. ZUTE03 with Novel Precursors Isolated from Tobacco Waste in a Two-Phase Conversion System
2011
Qiu, Lequan, Zhejiang University of Technology, Hangzhou, China | Wang, Weijian, Zhejiang University of Technology, Hangzhou, China | Zhong, Weihong, Zhejiang University of Technology, Hangzhou, China | Zhong, Li, Zhejiang University of Technology, Hangzhou, China | Fang, Jianjun, Zhejiang University of Technology, Hangzhou, China | Li, Xuanzhen, Zhejiang University of Technology, Hangzhou, China | Wu, Shijin, Zhejiang University of Technology, Hangzhou, China | Chen, Jianmeng, Zhejiang University of Technology, Hangzhou, China
Coenzyme Q∧10 (CoQ∧10) is a widely used supplement in heart diseases treatment or antioxidative dietary. The microbial production of CoQ∧10 was enhanced by addition of solanesol and novel precursors recovered from waste tobacco. The novel precursors were separated by silica gel and identified as α-linolenic acid (LNA) and butylated hydroxytoluene (BHT) based on the effect on CoQQ∧10 production and GC-MS. The effects of novel precursors on CoQ∧10 production by Sphingomonas sp. ZUTE03 were further evaluated in a two-phase conversion system. The precursor's combination of solanesol (70 mg/l) with BHT (30 mg/l) showed the best effect on the improvement of CoQ∧10 yield. A maximal CoQ∧10 productivity (9.5 mg l-¹ h-¹) was achieved after 8 h conversion, with a molar conversion rate of 92.6% and 92.4% on BHT and solanesol, respectively. The novel precursors, BHT and LNA in crude extracts from waste tobacco leaves, might become potential candidates for application in the industrial production of CoQ∧10 by microbes.
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