Kan tuat chua bacteria rokmai mansampalang chak bai lae din.
1988
Chalida Leksomboon | Niphone Thaveechai | Wichai Kositratana
Detection of Xanthomonas campestris pv. manihotis (Xcm) by the method of indirect immunofluorescent staining (IF) using antiserum produced in New Zealand White rabbit against membrane protein complex (MPC) of Xcm can be done at the minimum of 10(exp 2) cells/cm2 of leaf and 10(exp 3) cfu/g of amended soil. A semiselective SXM medium was developed for detection of Xcm containing (g/l): K2HPO4 0.8, KH2PO4 0.6, yeast extract 0.7, soluble starch 8.0, glucose 1, agar 15, methly green 0.01, methyl violet 2B 0.001, cephalexin 0.03, gentamicin sulfate 0.001, and cycloheximide 0.25. The medium showed 90-100 % inhibition of saprophytes from soil and can be used to detect Xcm from soil and leaf at minimum of 10(exp 3) cfu/g of soil and 10(exp 2) cfu/cm 2 of leaf, respectively. This experiment was the first report to demonstrate the use of IF and semiselective medium for detection of Xcm from leaf and soil. Efficiency of detection of the two methods was nearly the same which can be choosen either one when appropriated especially for epidemiological study.
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