Single-stranded DNA of 5'-upstream region of the rolC gene interacts with nuclear proteins of carrot cell cultures.
1992
Suzuki A. | Kato A. | Uchimiya H.
Using the gel retardation assay, proteins of carrot cells capable of binding to a single-stranded DNA of 5'-upstream region of the rolC gene were found. From competition experiments, these DNA protein interactions were specific to single-stranded nucleotide sequence of Ava S fragment (from -94 bp to +23 bp relative to the transcription initiation site). Methylation interference experiments showed that residue at the position of -41 bases on the bottom strand was important for DNA-protein binding. This residue was located between CAAT box and TATAA box. Such specific interaction between single-stranded DNA and nuclear proteins may play a role in transcription by RNA polymerase II.
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