Histological evaluation of ovine mammary tissue xenografted into cyclosporine-treated mice.
1991
Daniel T.E. | Akers R.M. | McFadden T.B.
Histological and pathological alterations of ovine mammary parenchyma xenografted into cyclosporine-treated mice were studied. Forty-two mature, virgin female mice were assigned randomly to one of four treatments: 1) control, 2) control + estrogen/progesterone, 3) cyclosporine, and 4) cyclosporine + estrogen/progesterone. All mice received two subcutaneous mammary tissue explants taken from an estrogen and progesterone-primed ewe. After 3, 7, 14, 21, and 35 d, mice were killed and tissue was removed for an evaluation of epithelial morphology on a scale from 1 (poor) to 5 (excellent). Leukocyte type and number were determined in subepithelial stroma. The mean epithelial score for tissue collected on d 7 and d 21 were higher (P < .05) for cyclosporine treatments (3.0) than for controls (1.6). Scores also were greater (P < .01) when cyclosporine was combined with estrogen and progesterone than when cyclosporine was the sole treatment (3.8 vs 2.3). Epithelial scores for tissue recovered from mice given cyclosporine and steroids up to 35 d were not different from those of explants fixed at zero time. However, scores were lower (P < .05) for three of five time periods for explants recovered from mice given cyclosporine alone. Lymphocyte number was higher (P < .07) in controls (66) than in mice given cyclosporine and steroids (13 per field). The correlation between lymphocyte number and epithelial score was -.55. These results indicate that ovine mammary tissue xenografted into mice treated with cyclosporine can maintain a normal histological structure for an extended period of time.
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