Application of mixed enzyme system using amylase and protease to cell wall determination and total digestible nutrients (TDN) of forages.
1994
Koga T. | Abe A.
This experiment has following two aims. First one is to develop a rapid and simple enzymatic method for the determination of feed cell wall. Second one is to develop an enzymatic digestion method having a high accuracy in TDN prediction of hay and silage. Enzymes, source organisms were alpha-amylase (Bacillus subtilis), actynase E (protease from Streptomyces griseus), panchdase HP (protease from Aspergillus oryzae) and cellulase for feed analysis (Trichoderma reesei). The materials examined here as feed samples for cell wall determination were five corn silages, sorghum silage, barley, wheat bran and fecal samples when corn silages were fed. Potato starch and soybean meal were supplied as standard samples of starch and protein. Cell wall materials such as xylan, cellulose, isolated cell wall derived from grass and legume were also used to determine the cellulase and hemicellulase activity of enzymes. Furthermore, sixty five forage (hays and silages) were used to TDN prediction. Samples containing starch gelatinized firstly and then incubated with enzyme solution for 16 hr. at 40 degrees C.
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