Kan chaknam hai koet yot chak cotyledonary node khong thua daengluang (Phaseolus vulgaris L.).
1992
Siranut Lamseejan | Thongchai Tonguthaisri | Siripong Kumpai
Experiment were carried out in order to compare three methods of multiple shoot formation and plantlet production in red kidney bean (Phaseolus vulgaris L.) All experiments utilized excised cotyledonary nodes from two week old seedling which were germinated on the medium containing BA. The highest number of shoots were produced on excised cotyledonary nodes which were cultured on the medium containing 5 mg/1 BA and 0.005 mg/l NNA. Elongated shoots from each treatments were rooted on the rooting medium to produce the complete plantlets. The highest number of complete plantlets were obtained from cotyledonary nodes which were cultured on the medium containing 0.05 mg/l BA and 0.005 mg/l NAA and 15 % coconut milk. In treating bean seeds with the gamma-rays at 50 Gy before germinating them on the medium containing BA, the number of shoots on the excised cotyledonary nodes was slightly decreased while the number of complete plantlet was slightly increased as compared with the controls.
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