A general suppressor of RNA polymerase 1, 2 and 3 mutations in Saccharomyces cerevisiae.
1993
Stettler S. | Chiannilkulchai N. | Hermann Le D.S. | Lalo D. | Lacroute F. | Sentenac A. | Thuriaux P.
A multicopy genomic library of Saccharomyces cerevisiae (strain FL100) was screened for its ability to suppress conditionally defective mutations altering the 31 kDa subunit (rpc31-236) or the 53 kDa subunit (rpc53-254/424) of RNA polymerase 3. In addition to allele-specific suppressors, seven suppressor clones were identified that acted on both mutations and also suppressed several other conditional mutations defective in RNA polymerases 1 or 2. All these clones harbored a complete copy of the SSD1 gene. The same pleiotropic suppression pattern was found with the dominant SSD1-v allele present in some laboratory strains of S. cerevisiae. SSD1-v was previously shown to suppress mutations defective in the SIT4 gene product (a predicted protein phosphatase subunit) or in the regulatory subunit of the cyclic AMP-dependent protein kinase. The SSD1 gene product modulates the activity (or the level) of the three nuclear RNA polymerases, possibly by altering their degree of phosphorylation.
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