Szarvasmarha-petesejtek in vitro termekenyitese.
1989
Schellander K. | Schellander E. | Fuhrer F. | Hauser C. | Schleger W. | Seregi J. | Peli J. | Treuer A. | Solti L. | Haraszti J. | Szasz F. | Benyei B. | Torok M.
After preliminary experiments carried out in sheep (1986), the first series of experiments have been reported on the in vitro fertilization (IVF) of a large number of bovine ova. Of 214 ovaria, collected on slaughterhouses, 421 ova were isolated. After the maturition in a growth medium and in vitro fertilization (using deep-frozen sperm cells, capacitated according to the method reported by Parrish et al., 1986), 271 (64.4 per cent) zygotes were trasferred onto the oviduet of sheep. After a 96 hours in vivo cultivation, 52 (19.2 per cent) formations were recovered. Of them 18 (34.6 per cent) embryos proved to be suitable for transfer onto bovine recipients. Of them 5 embryos were transferred (1x3 and 1x2, onto the uterine horns corresponding to the corpus luteum) onto heifers, synchronized by prostaglandin, according to the non-surgical method known from the literature. Although one of the recipients failed to show estrus and her serum progesterone level was also high on the 14th day, the procedure did not result in pregnancy.
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