The use of markers to select somatic hybrids through protoplast fusion in Oriza sativa L.
1988
Lee H.Y. | Kameya Toshiaki | Lee J.S.
During the last decade, many somatic hybrids were obtained through protoplast fusion. However, the process of somatic hybridization through protoplast fusion poses some problems in establishing an effective method of selecting real hybrid plants or colonies from the fused cells. In this study, we report that hybrid rice plants were successfully obtained by protoplast fusion between 2 cultivars, Murasakidaikoku and Yamahoshi. Murasakidaikoku has deep anthocyanin color determined by a dominant gene, and its plant type is dwarf one determined by a recessive gene. Moreover, the Murasakidaikoku callus derived from anther culture had lost plant regeneration ability because of long period for subculture, while Yamahoshi cells derived from anther culture had good plant regeneration ability at the time of present experiment. Therefore, regenerated plants having anthocyanin color would be highly suspected to be somatic hybrids. From this experiment, 2 plantlets were obtained which had light anthocyanin color with shoots and their chromosome number were 24. These results indicate that these plants were somatic hybrids through protoplast fusion, and anthocyanin coloring and plant regeneration ability of Murasakidaikoku are efficient selection markers in somatic hybridization.
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