Device for field measurement of conidia release by a single leaf blast lesion.
1988
Chang Kyu Kim | Reiichi Yoshino
In the study of the life cycle of rice blast fungus Pyricularia oryzae, measurement under field conditions of the conidia released from a single lesion is important in understanding conidia dispersal pattern. Some preliminary experiment to improve spore catch efficiency were done during the 1985 crop season in Korea. Using those results, a new KY type spore trap was devised. A discrete lesion-bearing leaf is fixed with double-sided adhesive tape on the acrylic rain protector without detaching the leaf from the plant. A basin-shaped acrylic capsule 40 mm long is placed just beneath the lesion. Collection is at 1- or 24-h intervals. Spores can be counted immediately or the capsule can be sealed with masking tape and the spores counted a few weeks or months later. When spores are counted 0.1 ml of 0.02% Tween 20 solution are added to the capsule and the conidia counted with a hemacytometer. Spores in one capsule can be counted in less than 10 min. The amount varies, depending upon the condition of the lesion as well as on the weather. The maximum number exceeded 30,000 conidia/d, suggesting the usefulness of a KY type spore trap to study conidia release under natural conditions.
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