Chlorophyll meter (SPAD-501) to quantify relative cold tolerance in rice [Philippines].
1986
Jiang Xian Xiang | Vergara B.S.
The results indicate that temperature during measurement did not affect chlorophyll reading, suggesting that SPAD-501 can be used under high or low temperatures. Leaf samples were taken from plants having different degrees of yellowing. The chlorophyll content was extracted by dimethyl-sulfoxide (DMSO) method. There was a very high correlation (r = 0.989** ) between SPAD-501 reading and chlorophyll content. The instrument manufacturer obtained a correlation of 0.815 between chlorophyll reading and actual chlorophyll content using data from several crop species. SPAD-501 is reliable in determining relative chlorophyll content as an index of cold tolerance. This method has considerable potential in quantifying leaf discoloration due to low temperature as well as yellowing caused by other stresses such as nutrient deficiency, diseases, and drought.
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