Development of a mathematical model for estimation of the tryptophan content in peptides.
1995
Meyer K. | Steinhart H. | Vollmar M.
A mathematical model for the estimation of the tryptophan (Trp) content in oxidized peptides was developed. Analytical parameters were the peptide recovery (determined by HPLC) and the recovery of the neighboring amino acids (determined by GC). The correlation of the peptide recovery with the degradation of the neighboring amino acids was derived by using a model and was applied to calculate the Trp recovery rate. The ratio of the degradation of Trp and the degradation of the neighboring amino acids in the peptides was estimated and defined as a factor named the stability factor. Equations describe the Trp reduction in the model peptides in dependence on the peptide recovery and the respective stability factors.
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