The level of abutilon mosaic geminivirus in leaf discs and wound callus.
1994
Song J.Y. | Jeske H.
The level of Abutilon mosaic geminivirus in leaf discs, wound callus and suspension culture was monitored by hybridization. Over 25 days the amount of AbMV DNA decreased in the leaf discs kept under various experimental conditions including different temperatures, light regimes and hormone concentrations. In primary callus or in suspension culture no viral DNA was detectable. When leaf discs were kept in liquid medium instead of on agar plates a shift from viral single-stranded DNA to replicative double-stranded DNA was observed when phytohormones were supplied. It is discussed here why under these conditions, rapidly diving tissues seem to be virus-free.
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