Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp., KGG32
2011
Oskay, M. (Celal Bayar Univ., Manisa (Turkey). Biology Dept.)
The purpose of the present study was concerned with the taxonomy and influences of some cultural conditions on growth and antimicrobial metabolite production of a strain of Streptomyces, designated KGG32, which is a highly stable antimicrobial metabolite producer against the Gram-positive and Gram-negative bacteria under in-vitro growth conditions. In order to optimize the culture conditions for the production of antimicrobial metabolite, such as the effect of different temperatures, nature of carbon and nitrogen sources, pH value and time incubation were determined. Effects of nutritional compounds on production of antimicrobial compounds showed that the highest antimicrobial activities were obtained when sucrose at 1.0% (w/v) level was used as sole carbon source, under aerobic conditions at temperature 30 degree C, pH at 7.5. Bacteriological peptone was identified as nitrogen source that significantly affected antibiotic production followed by meat extract. Phenotypic characters of the strain strongly suggested that this strain belongs to the genus Streptomyces. The strain was grey colored with rectiflexibiles spore chains, exhibited resistance to neomycin, vancomycin and penicillin. It had the ability to produce enzymes such as caseinase amylase and xylanase. The results showed that strain KGG32 was a potential soil microorganism with antimicrobial activity and may be used for biotechnological purposes.
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