Synergism between Bacillus thuringiensis toxins Cry1Ac and Cry2Aa against Earias vitella (Lepidoptera)
2011
Yunus, F.N. (Lahore Coll. for Women Univ. (Pakistan)) | Makhdoom, R. (Punjab Univ., Lahore (Pakistan). Centre of Excellence in Molecular Biology) | Raza, G. (Punjab Univ., Lahore (Pakistan). Centre of Excellence in Molecular Biology)
Most strains of insect killing bacterium Bacillus thuringiensis have a combination of different toxins in their parasporal crystals. Some of the combinations clearly interact synergistically like the toxins present in Bt. subspecies israelensis. The present study reveals that a mixture of different toxins could be more effective than a single toxin. In this study, the possible synergistic effects between two Cry toxins i.e. Cry1Ac (CAMB 10227) and Cry2Aa (CAMB 10082) on Earias vitella (Spotted bollworm) the major pest for cotton in three different combinational ratios of toxins i.e., 1:1, 1:2, 2:1 was observed through artificial diet bioassays. Expected toxicity was calculated from the activity of each individual toxin and its proportion in the combinations. Fifty percent mortality was calculated from biotoxicity experiments by probit analysis. The results showed the synergism significantly in three different Cry toxins combinational ratios.
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