Interaction of ghrelin and opioids in luteinizing hormone (LH) secretion by pituitary cells of common carp, Cyprinus carpio (Actinopterygii: Cypriniformes: Cyprinidae), cultured in vitro
2011
Sokolowska-Mikolajczyk, M. | Socha, M. | Mikolajczyk, T. | Epler, P. | Falowska, B.,Krakow Agricultural University (Poland). Dept. of Ichthyobiology and Fisheries
Ghrelin, a newly discovered hormone is involved mainly in the regulation of body energy homeostasis. In the in vitro experiment the time-dependent (10 and 24 h) action of ghrelin (10_7 or 10_6 M) and naltrexone at 10_6 M added alone or in combination on LH secretion by enzymatically dispersed pituitary cells of mature female and male carp (Cyprinus carpio L.) was studied. Ghrelin alone at a dose of 10_6 M stimulated LH secretion after 10 h of female- but not male-derived cell incubations. Ghrelin at 10_7 M had no effect on LH secretion from either female or male cells. Naltrexone alone stimulated LH secretion only after 24 h of female cell culture. In male cells no significant changes in LH secretion in response to naltrexone alone were found after 10 or 24 h incubation period. Combined treatment (ghrelin and naltrexone) stimulated LH secretion in female and male cell incubations at 10 and 24 h: LH levels were significantly higher in comparison to control, to ghrelin alone (in cells of both sexes) and to naltrexone alone (in male cells only). The results suggest that opioids and ghrelin may control LH secretion in carp acting synergistically, probably through the same receptor type
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