Serologic investigation of activity of west Nile virus in humans in three district of Vojvodina
2012
Hrnjaković-Cvjetković, I., Institute for Public Health of Vojvodina, Novi Sad (Serbia) | Milošević, V., Institute for Public Health of Vojvodina, Novi Sad (Serbia) | Jerant-Patić, V., Institute for Public Health of Vojvodina, Novi Sad (Serbia) | Petrović, T., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Petrić, D., Faculty of Agriculture, Novi Sad (Serbia) | Stefan-Mikić, S., Clinical Center of Vojvodina, Novi Sad (Serbia) | Zgomba, M., Faculty of Agriculture, Novi Sad (Serbia)
The aim of this study was to investigate the presence of West Nile virus specific antibodies in the serum samples from three districts of Vojvodina using IgG ELISA assay. During the period 2007-2010, 426 serum samples were collected from residents of South Backa District. During September and October 2011, 91 serum samples were collected from residents of central Banat and North Backa District. All samples were tested for West Nile Virus IgG by commercial enzyme-linked immunosorbent assay (ELISA) test, produced by Euroimmun, Luebeck, Germany in Institute of Public Health of Vojvodina. Testing, calculation and interpretation of results were performed strictly following the manufacturers instructions on the automatic device Euroimmun Analyzer I-2P. X square test was used for statistic calculations. In the serosurveys, conducted during period 2007-2010 in South Backa District, IgG antibody against WN virus was found in 16 (3.75%) out of 426 serum samples. In the study conducted in 2011 in North Backa District IgG antibody to West Nile virus was found in 1 out of 43serum samples (2.32%). In samples from Central Banat seroprevalence of WNV IgG antibody was 12.5% (6/48) that was significantly higher than seroprevalence for two other district (X**2 = 13.07, p is less than 0.01). According to the results of this study the virus was present in all investigated districts and circulates among humans.
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