Discrimination of the production system of lamb meat through the fatty acid composition of muscle
2011
Alves, S.P., Instituto Nacional dos Recursos Biológicos-INRB, Fonte Boa (Portugal). Unidade de Produção Animal | Santos-Silva, J., Instituto Nacional dos Recursos Biológicos-INRB, Fonte Boa (Portugal). Unidade de Produção Animal | Bessa, R.J.B., Instituto Nacional dos Recursos Biológicos-INRB, Fonte Boa (Portugal). Unidade de Produção Animal
Forty Merino Branco ram lambs were used to study the effect of four feeding systems. Lambs were randomly assigned to four feeding systems as follow: pasture for six weeks (PPP); four weeks of pasture feeding followed by two weeks of finishing on concentrate (PPC); two weeks of pasture feeding followed by four weeks of finishing on concentrate (PCC); four weeks on concentrate only (CCC). At the end of the trial, lambs were slaughtered, carcasses were stored at 2 deg C for 72h and after that samples from the longissimus dorsi muscle were collected, minced, vacuum packed, freeze-dried and stored at -80 deg C until lipid analysis. Lipids from muscle samples were extracted and converted to fatty acid methyl esters by base-catalyzed transesterification. Fatty acid methyl esters were analyzed by gas-liquid chromatography and fatty acid composition data were subjected to analysis of variance, considering the different feeding systems as a single effect. Canonical discriminant analysis (CDA) was applied to fatty acid data in order to classify and predict the feeding system. From the 53 fatty acids identified in muscle samples, only 36 were affected by the production system, including the major 18:1cis-9, 16:0, 18:0, 18:3n-3, 18:2cis-9,trans-11 (CLA). The recognition ability of the discriminant model was evaluated by the correct classification of 100% during the modeling step, allowing the differentiation of the four feeding systems.
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