Characterization of a carbendazim-degrading Variovorax paradoxus strains
2011
Manczinger, L., University of Szeged,Szeged (Hungary). Faculty of Science and Informatics, Department of Microbiology | Németh, V., University of Szeged, Szeged (Hungary). Faculty of Science and Informatics, Department of Physical Chemistry | Sajben, E., University of Szeged,Szeged (Hungary). Faculty of Science and Informatics, Department of Microbiology | Škrbić, B., Faculty of Technology, Novi Sad (Serbia) | Krisch, J., University of Szeged, Szeged (Hungary). Institute of Food Engineering | Vágvölgyi, Cs., University of Szeged,Szeged (Hungary). Faculty of Science and Informatics, Department of Microbiology
One of the most frequently used fungicides is carbendazim, however, in spite of its importance, there are only a few reports dealing with its microbial degradation in the environment. It has high acute ecotoxicological effect, as well as a suspected endocrine disruptor potential, so its residues in food and feed are dangerous. Until now, single isolates Pseudomonas, Rhodococcus and Ralstonia have been found to be able to degrade carbendazim. Among fungi, one isolate of Alternaria alternata and Phanaerochete crysosporium were described as good carbendazim degrades. As part of our studies on pesticide biodegradation, new carbendazim-metabolizing bacteria were isolated from Hungarian agricultural soil samples. These degrader bacteria were isolated from soil samples by microbiological enrichment methods. The molecular analysis revealed that the best isolates belong to the Variovorax paradoxus species. the isolate 10/1 was able to use carbendazim as sole carbon and nitrogen source. The pH optimum and temperature optimum for growth were found to be pH 6.3 and 30 deg C, respectively. The presence of yeast extract at low concentration (150 mg/l) highly improved the degradation activity. The glucose in the presence of ammonium ions fully repressed the carbendazim-degrading activity of the strain. This isolate seems to be an efficient tool for the bioremediation of carbendazim polluted agricultural soils.
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