The cytotoxic effect of essential oils Citrus aurantium peels on human colorectal carcinoma cell line (LIM1863)
2012
Odeh, F., Damascus University (Syria). Faculty of Pharmacy, Pharmacognosy, Deparment | Rahmo, A. | Alnori, A. S. | Chaty, M. E.
Citrus essential oils (EOs) contain different terpens that have been shown to possess antitumor effects. We determined the cytotoxic effect of essential oils of Citrus aurantium L. subspamara peels on a colorectal cancer cell line (Lim1863). Three samples were harvested from three locations in Syria. EOs were extracted by hydrodistilation and analyzed by GC-MS. EOs content of Limonene was 96-97.7% while alpha-pinene and beta-myrcene were 0.35-1% and 0.9-1.4% respectively. Various concentrations of EOs (0.25-48 micro l per ml) were added to cultured cells and incubated for 72 h. Cell viability was evaluated using the MTT-based cytotoxicity assay. The obtained IC50 value range of C. aurantium Eos was 2.18-2.44 micro l per ml. In conclosion, C. aurantium peels Eos obviously reduced the cell viability and it might have cytotoxic effect against colorectal cancer cell line.
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