Effect of macrolide antibiotics on various cell cultures in vitro: 1. Cell morphology
2012
Fülöpová, D. | Kováčik, A., Slovak Univ. of Agriculture in Nitra (Slovak Republic). Faculty of Biotechnology and Food Sciences, Department of Animal Physiology | Kováčová, R. | Čupka, P. | Massányi, P.
The aim of our study was to evaluate the cytotoxicity of macrolide antibiotics (tilmicosin, tylosin and spiramycin) of various concentrations on different cell cultures in vitro. Cellular lines from animal tissues (VERO cells - kidney cells of Macacus rhesus, FE cells - feline embryonal cells, BHK 21 cellular line from young hamster kidneys) were used. Tilmicosin effect: BHK cells are most sensitive, significant decrease in vital cells occurs already at the concentration of 50 micro g per ml. VERO cells were most resistant, significant decrease of vital cells was observed only at the concentration of 300 micro g per ml. Tylosin effect: BHK cells can be considered most sensitive, since at concentrations higher than 500 micro g per ml, no vital cells were observed. At the concentration of 1000 micro g per ml were 3.13% of vital and 70.52% of subvital FE cells. In Vero cells, we observed a significant decrease at the concentration of 750 micro g per ml. Spiramycin effect: Significant decrease of vital BHK cells was observed at the concentration of 150 micro g per ml, at the concentration of 300 micro g per ml, no vital cells and only 7.53% of subvital cells were observed. At the concentration of 500 micro g per ml reported 10.34% of vital FE cells. At the concentration of 500 micro g per ml 22.48% of vital and 71.16% of subvital VERO cells were recorded.
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