The effect of curcumin on secretory activity, proliferation and apoptosis of the porcine ovarian granulosa cells
2012
Kádasi, A., Slovak Univ. of Agriculture in Nitra (Slovak Republic). Faculty of Biotechnology and Food Sciences, Department of Animal Physiology | Sirotkin, A.V., Animal Production Research Centre, Nitra (Slovak Republic) | Maruniaková, N. | Kolesárová, , A. | Bulla, J. | Grossmann, R., Institute of Animal Genetics, Neustadt (Germany). Department of Functional Genomics and Bioregulation
The aim of this in vitro study was to examine the effect of natural plant (Curcuma longa) molecule curcumin on secretory activity, proliferation and apoptosis of porcine granulosa cells. The secretion of steroid hormones progesterone, testosterone), accumulation of PCNA (marker of proliferation) and bax (marker of apoptosis) in granulosa cells of swine ovaries after curcumin treatment at the doses 0, 1, 10, 100 micro g per mL was determined by RIA and immunocytochemistry. It was observed that, addition of curcumin stimulated progesterone (at doses 1 and 10 micro g per mL, but not 100 micro g per mL) and testosterone at (100 micro g per mL but not 1 and 10 micro g per mL) release. The number of cells contained PCNA was down-regulated by curcumin administration (at dose of 10 micro g per mL, but not of 1 and 100 micro g per mL). Bax expression was stimulated by curcumin at all doses added. Our results suggest a direct effect of curcumin on ovarian functions: steroidogenesis, proliferation and apoptosis. This could suggest antireproductive properties of curcumin in swine ovaries.
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